Structural basis of RNA-guided transcription by a dCas12f–σ^E–RNAP complex

Nature, Published online: 04 March 2026; doi:10.1038/s41586-026-10178-3

Cryogenic electron microscopy reveals how dCas12f with σE recruits RNAP to targeted DNA, initiating transcription at a fixed downstream distance, bypassing canonical −35 recognition and stabilizing the −10 element in an unusual manner.